Overview

  • Product name
  • Description
    Goat polyclonal to IRE1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    C-PNAHGKIKAMISD

    , corresponding to internal sequence amino acids 699-711 of human IRE1 (NP_001424.3).

  • Positive control
    • Human pancreas lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab77544 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • Tissue specificity
      Ubiquitously expressed. High levels observed in pancreatic tissue.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 antibody images

    • Anti-IRE1 antibody (ab77544) at 0.03 µg/ml + human pancreas lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 110 kDa

    References for Anti-IRE1 antibody (ab77544)

    This product has been referenced in:
    • Xue LX  et al. Neuroprotective effects of Activin A on endoplasmic reticulum stress-mediated apoptotic and autophagic PC12 cell death. Neural Regen Res 12:779-786 (2017). WB . Read more (PubMed: 28616035) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab77544.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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