Overview

  • Product nameAnti-IRE1 antibody
    See all IRE1 primary antibodies
  • Description
    Mouse monoclonal to IRE1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP , corresponding to amino acids 401-500 of Human IRE1 (AAC25991) with a 26 kDa tag

  • Positive control
    • HeLa cell lysate, Human esophagus tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab87964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1.5 µg/ml. Antigen retrieval is not essential but may optimise staining when the microwave method is used.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSenses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • Tissue specificityUbiquitously expressed. High levels observed in pancreatic tissue.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localizationEndoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 antibody images

    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + Immunogen (Recombinant protein) at 0.2 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + HeLa cell lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
    • ab87964 at 1.5µg/ml staining IRE1 in Human esophagus by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    References for Anti-IRE1 antibody (ab87964)

    ab87964 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab87964.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"