Recombinant
RabMAb

Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

Overview

  • Product name
    Anti-IRE1 (phospho S724) antibody [EPR5253]
    See all IRE1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5253] to IRE1 (phospho S724)
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human IRE1 aa 700-800 (phospho S724). The exact sequence is proprietary.
    Database link: O75460

  • Positive control
    • Calyculin A treated 293T cell lysate
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.10 x 10 -10 M Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR5253
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • Tissue specificity
      Ubiquitously expressed. High levels observed in pancreatic tissue.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localization
      Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 (phospho S724) antibody [EPR5253] images

    • All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/5000 dilution

      Lane 1 : K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : K562 (Human chronic myelogenous leukemia lymphoblast) treated with tunicamycin at 5ug/ml for 30 minutes. Whole cell lysates.
      Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast) treated with tunicamycin at 5ug/ml for 30 minutes. Whole cell lysates.Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 110 kDa
      Observed band size : 110 kDa


      Exposure time : 3 seconds

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Dot Blot analysis of Lane 1: IRE1 (pS724) phospho peptide and Lane 2: IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time: 3 minutes.

    • All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/2000 dilution (purified)

      Lane 1 : K562 cell lysate treated with phosphatase
      Lane 2 : Untreated K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size : 110 kDa
      Observed band size : 110 kDa

      Dilution buffer: 2% BSA/TBST

      Blocking buffer: 2% BSA/TBST

    • All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/5000 dilution

      Lane 1 : K562 (human chronic myelogenous leukemia) treated with Calyculin A whole cell lysates
      Lane 2 : K562 (human chronic myelogenous leukemia) treated with Calyculin A whole cell lysates plus IRE1 phospho S724 peptide
      Lane 3 : K562 (human chronic myelogenous leukemia) treated with Calyculin A whole cell lysates plus IRE1 non-phospho peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit IgG, (H+L), HRP conjugated (ab97051) at 1/20000 dilution

      Predicted band size : 110 kDa
      Observed band size : 110 kDa


      Exposure time : 15 seconds

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/1000 dilution

      Lane 1 : Untreated 293T cell lysate
      Lane 2 : Calyculin A treated 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution
      Developed using the ECL technique

      Predicted band size : 110 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945)

    This product has been referenced in:

    See all 10 Publications for this product

    Product Wall

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Loading amount
    30 µg
    Treatment
    10 ´M tunicamycin for 30 min, 1h, 2h
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tamas Czompoly

    Verified customer

    Submitted May 19 2017

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293 cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    15 µg
    Treatment
    25 µM Cadmium for 12h
    Specification
    HEK293 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 10 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (HeLa cells)
    Loading amount
    40000 cells
    Specification
    HeLa cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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