Overview

  • Product nameAnti-IREB2 antibody
    See all IREB2 primary antibodies
  • Description
    Rabbit polyclonal to IREB2
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Pig, Caenorhabditis elegans, Zebrafish
  • Immunogen

    Synthetic peptide: QVIQINLNSI VPSVSGPKRP QDRVAVTDMK SDFQACLNEK VGFKGFQIAA , corresponding to amino acids 432-481 of Human IREB2

  • Positive control
    • Fetal liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • FunctionRNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
  • Sequence similaritiesBelongs to the aconitase/IPM isomerase family.
  • Post-translational
    modifications
    Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACO3 antibody
    • FLJ23381 antibody
    • IRE BP2 antibody
    • IRE-BP 2 antibody
    • IREB 2 antibody
    • IREB2 antibody
    • IREB2_HUMAN antibody
    • Iron regulatory protein 2 antibody
    • iron responsive element binding protein 2 antibody
    • Iron-responsive element-binding protein 2 antibody
    • IRP 2 antibody
    • IRP2 antibody
    • IRP2AD antibody
    • OTTHUMP00000185022 antibody
    see all

Anti-IREB2 antibody images

  • Anti-IREB2 antibody (ab80339) at 1 µg/ml + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa

References for Anti-IREB2 antibody (ab80339)

ab80339 has not yet been referenced specifically in any publications.

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