Anti-IRF1 antibody (ab191032)
Key features and details
- Rabbit polyclonal to IRF1
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IRF1 antibody
See all IRF1 primary antibodies -
Description
Rabbit polyclonal to IRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IRF1 aa 150-250 (internal sequence).
Database link: P10914 -
Positive control
- COLO320, U87, HeLa, Jurkat and MCF-7 cell lysates; Human intestinal cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 36 kDa.
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ICC |
Use a concentration of 0.5 - 1 µg/ml.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 36 kDa. |
ICC
Use a concentration of 0.5 - 1 µg/ml. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor. -
Involvement in disease
Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Post-translational
modificationsSumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3659 Human
- Omim: 147575 Human
- SwissProt: P10914 Human
- Unigene: 436061 Human
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Alternative names
- Interferon regulatory factor 1 antibody
- Interferon regulatory factor 1 isoform +I9 antibody
- Interferon regulatory factor 1 isoform d78 antibody
see all
Images
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All lanes : Anti-IRF1 antibody (ab191032) at 0.5 µg/ml
Lane 1 : COLO320 cell lysate
Lane 2 : U87 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF-7 cell lysate
Predicted band size: 36 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human intestinal cancer tissue labeling IRF1 with ab191032 at 1 μg/ml.
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Immunocytochemical analysis of HeLa cells labelling IRF1 with ab191032 at 1μg/ml
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab191032 has been referenced in 3 publications.
- Zeng Z et al. Overexpression of OASL upregulates TET1 to induce aberrant activation of CD4+ T cells in systemic sclerosis via IRF1 signaling. Arthritis Res Ther 24:50 (2022). PubMed: 35183246
- Cobo I et al. Monosodium urate crystals regulate a unique JNK-dependent macrophage metabolic and inflammatory response. Cell Rep 38:110489 (2022). PubMed: 35263587
- Koelzer VH et al. Digital analysis and epigenetic regulation of the signature of rejection in colorectal cancer. Oncoimmunology 6:e1288330 (2017). PubMed: 28507795