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Product name
Anti-IRF1 antibody - ChIP Grade
See all IRF1 products (3) ...
Description
Rabbit polyclonal to IRF1 - ChIP Grade
Tested applications
IHC-P, ICC/IF, WB, Flow Cyt, ChIPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 225 - 325 of Human IRF1.
(Peptide available as ab277 63.)
Positive control
MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab26109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/225. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt: Use at an assay dependent concentration. PubMed: 21411754
ChIP: Use at an assay dependent concentration. PubMed: 21757724
Target
Function
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.
Involvement in disease
Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Post-translational
modifications
Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP10914
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- interferon regulatory factor 1 antibody
- Interferon regulatory factor 1 antibody
- IRF 1 antibody
see all
Database links
- Entrez Gene: 3659 Human
- Omim: 147575 Human
- SwissProt: P10914 Human
- Unigene: 436061 Human
Anti-IRF1 antibody - ChIP Grade images:
Western blot - IRF1 antibody (ab26109)
Anti-IRF1 antibody - ChIP Grade (ab26109) at 1 µg/ml +
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 36 kDa
Observed band size : 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF1 antibody (ab26109)
Image courtesy of Human Protein Atlas. ab26109 staining IRF1 in human duodenum, showing nuclear staining of glandular cells. Paraffin embedded human duodenum tissue was incubated with ab26109 (1/225 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26109 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
Immunocytochemistry/ Immunofluorescence - IRF1 antibody (ab26109)
ICC/IF image of ab26109 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab26109, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
References for Anti-IRF1 antibody - ChIP Grade (ab26109)
This product has been referenced in:
- Schoggins JW et al. A diverse range of gene products are effectors of the type I interferon antiviral response. Nature : (2011). ICC/IF; Human.Read more (PubMed: 21478870) »
- Harman AN et al. HIV infection of dendritic cells subverts the IFN induction pathway via IRF-1 and inhibits type 1 IFN production. Blood 118:298-308 (2011). Flow Cyt; Human.Read more (PubMed: 21411754) »
See all 4 publications for this product
Publishing research using ab26109? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"