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Synthetic peptide conjugated to KLH derived from within residues 225 - 325 of Human IRF1.
(Peptide available as ab27763.)
Our Abpromise guarantee covers the use of ab26109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/225. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 21411754ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|ChIP||Use at an assay dependent concentration. PubMed: 21757724|
Image courtesy of Human Protein Atlas. ab26109 staining IRF1 in human duodenum, showing nuclear staining of glandular cells. Paraffin embedded human duodenum tissue was incubated with ab26109 (1/225 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab26109 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org
ICC/IF image of ab26109 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab26109, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.
HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin fragments were subject to ChIP using ab26109 (anti-IRF1) or a control (normal IgG). DNA from the immunoprecipitates was analyzed by qRT-PCR using primers specific for the miR-203 promoter.
Data from: PLoS One (2015) 10(2), e0117035. Mao L, Zhang Y, Mo W, Yu Y and Lu H. BANF1 is downregulated by IRF1-regulated microRNA-203 in cervical cancer.
This image is courtesy of an anonymous Abreview
Flow cytometric analysis of paraformaldehyde-fixed, Triton X-100 permeabilized human macrophages labeling IRF1 with ab26109 at 1/200 dilution (red) compared with an ssotype control details (black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"