Overview

  • Product nameAnti-IRF2 antibody
    See all IRF2 primary antibodies
  • Description
    Goat polyclonal to IRF2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    Synthetic peptide:

    KTSDITQARVKSC

    , corresponding to C terminal amino acids 337-349 of Human IRF2.

  • Positive control
  • General notesPrincipal Names – IRF2; IRF-2; interferon regulatory factor 2 Official Gene Symbol - IRF2 GenBank Accession Number – NP_002190 LocusLink ID - 3660 (human); 16363 (mouse) Gene Ontology terms - RNA polymerase II transcription factor activity; cell proliferation; oncogenesis; negative regulation of transcription from Pol II promoter

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human IRF2 peptide (ab22943). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295.

Target

  • FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
  • Tissue specificityExpressed throughout the epithelium of the colon. Also expressed in lamina propria.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
    The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686F0244 antibody
    • Interferon regulatory factor 2 antibody
    • IRF 2 antibody
    • IRF-2 antibody
    • IRF2 antibody
    • IRF2_HUMAN antibody
    see all

Anti-IRF2 antibody images



  • Predicted band size : 38 kDa


    ab3388 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    The observed molecular weight corresponds to earlier findings in literature with different antibodies (Li et al, Invest Ophthalmol Vis Sci. 1999 Apr;40(5):976-82.; PMID: 10102295.

References for Anti-IRF2 antibody (ab3388)

This product has been referenced in:
  • Liljeroos M  et al. Bacterial ligand of TLR2 signals Stat activation via induction of IRF1/2 and interferon-alpha production. Cell Signal 20:1873-81 (2008). Read more (PubMed: 18652890) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. All the information that we have about this antibody is contained on the antibody datasheet. To clarify this antibody has not been tested during developmental stages. This antibody is a mixture of many different goat...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"