The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 39 kDa.
Use a concentration of 3 µg/ml.
Use a concentration of 10 µg/ml.
FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
Tissue specificityExpressed throughout the epithelium of the colon. Also expressed in lamina propria.
Sequence similaritiesBelongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Post-translational modificationsAcetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity. The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
Lane 1: Wild type HAP1 whole cell lysate (20 µg) Lane 2: IRF2 knockout HAP1 whole cell lysate (20 µg) Lane 3: HeLa whole cell lysate (20 µg) Lane 4: CACO2 whole cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab55333 observed at 48 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab55333 was shown to specifically react with IRF2 when IRF2 knockout samples were used. Wild-type and IRF2 knockout samples were subjected to SDS-PAGE. Ab55333 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
IHC-P - IRF2 antibody (ab55333)
IRF2 antibody (ab55333) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human leiomyosarcoma.
Western blot - IRF2 antibody (ab55333)
Predicted band size : 39 kDa IRF2 antibody (ab55333) at 1ug/lane + HeLa cell lysate at 25ug/lane.