Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab86018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 39 kDa.

Target

  • FunctionSpecifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation.
  • Tissue specificityExpressed throughout the epithelium of the colon. Also expressed in lamina propria.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity.
    The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686F0244 antibody
    • Interferon regulatory factor 2 antibody
    • IRF 2 antibody
    • IRF-2 antibody
    • IRF2 antibody
    • IRF2_HUMAN antibody
    see all

Anti-IRF2 antibody images

  • All lanes : Anti-IRF2 antibody (ab86018) at 1/1000 dilution

    Lane 1 : Uninduced (negative control) culture of E.coli
    Lane 2 : Induced culture of E.coli

    Secondary
    anti-Rabbit IgG HRP conjugated at 1/1000 dilution

    Predicted band size : 39 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,55 kDa,60 kDa. We are unsure as to the identity of these extra bands.

References for Anti-IRF2 antibody (ab86018)

ab86018 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"