IRF2 overexpression 293T lysate (whole cell) (ab94267)

Overview

  • Product nameIRF2 overexpression 293T lysate (whole cell)
  • Description
    IRF2 overexpression lysate
  • General notesab94267 is a 293T cell transfected lysate in which Human IRF2 has been transiently over-expressed using a pCMV-IRF2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. PTM: Acetylated by CBP/ p300 during cell-growth. Acetylation on Lys-75 is required for stimulation of H4 promoter activity. The major sites of sumoylation are Lys-137 and Lys-293. Sumoylation by SUMO1 increases its transcriptional repressor activity on IRF1 and diminishes its ability to activate ISRE and H4 promoter. Similarity: Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain. Tissue specificity: Expressed throughout the epithelium of the colon. Also expressed in lamina propria.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

IRF2 overexpression 293T lysate (whole cell) images

  • ab94267 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-IRF2 antibody (ab55331) at 1/500 dilution

    Lane 1 : IRF2 overexpression 293T lysate (whole cell) (ab94267)
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for IRF2 overexpression 293T lysate (whole cell) (ab94267)

ab94267 has not yet been referenced specifically in any publications.

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