Overview

  • Product nameAnti-IRF3 antibody [5D2]
    See all IRF3 primary antibodies
  • Description
    Mouse monoclonal [5D2] to IRF3
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant Human IRF3 protein expressed in 293T cells.

  • Positive control
    • IRF3-transfected HEK293T cell lysate; HeLa, HT29, COS7 and MCF7 cell extracts; Human breast, breast adenocarcinoma, colon adenocarcinoma, kidney carcinoma, lung, Ovary, ovary adenocarcinoma, pancreas, pancreas carcinoma, thyroid, thyroid carcinoma, endometrium, prostate carcinoma and bladder carcinoma tissues
  • General notesDilute in PBS (pH7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab124399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 47 kDa.
IHC-P 1/50.

Target

  • FunctionMediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
  • Tissue specificityExpressed constitutively in a variety of tissues.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
    Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
    ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interferon regulatory factor 3 antibody
    • IRF 3 antibody
    • IRF-3 antibody
    • IRF3 antibody
    • IRF3_HUMAN antibody
    • MGC94729 antibody
    see all

Anti-IRF3 antibody [5D2] images

  • All lanes : Anti-IRF3 antibody [5D2] (ab124399) at 1/2000 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY IRF3

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 47 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC209951)
  • All lanes : Anti-IRF3 antibody [5D2] (ab124399) at 1/2000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 47 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC209951)
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human breast tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human breast adenocarcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human kidney carcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human lung tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human overy tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human overy adenocarcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human pancreas tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human pancreas carcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human thyroid tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human endometrium tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
  • ab124399 at 1/50 dilution staining IRF3 in Paraffin-embedded Human bladder carcinoma tissue by Immunohistochemistry.

References for Anti-IRF3 antibody [5D2] (ab124399)

ab124399 has not yet been referenced specifically in any publications.

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