Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)


  • Product nameAnti-IRF3 (phospho S386) antibody [EPR2346]
    See all IRF3 primary antibodies
  • Description
    Rabbit monoclonal [EPR2346] to IRF3 (phospho S386)
  • SpecificityDetects IRF3 when phosphorylated on Serine 386.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho-specific peptide corresponding to residues surrounding Serine 386 of Human IRF3

  • Positive control
    • MCF-7 cells treated with Calyculin A.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-IRF3 antibody (Alexa Fluor® 488) [EPR2346] (ab193243)
    Anti-IRF3 antibody (Alexa Fluor® 647) [EPR2346] (ab192981)



Our Abpromise guarantee covers the use of ab76493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • FunctionMediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
    • Tissue specificityExpressed constitutively in a variety of tissues.
    • Sequence similaritiesBelongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • Post-translational
      Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
      Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
      ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation.
    • Cellular localizationCytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • IIAE7 antibody
      • Interferon regulatory factor 3 antibody
      • IRF 3 antibody
      • IRF-3 antibody
      • IRF3 antibody
      • IRF3_HUMAN antibody
      • MGC94729 antibody
      see all

    Anti-IRF3 (phospho S386) antibody [EPR2346] images

    • All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 1/20000 dilution

      Lane 1 : MCF7 cell lysate - untreated
      Lane 2 : MCF7 cell lysate – treated with Calyculin A

      Lysates/proteins at 20 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size : 47 kDa

      Blocking buffer - 5% NFDM/TBST

      Diluting buffer - 1% BSA

    • Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.

    References for Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)

    This product has been referenced in:
    • Huang LY  et al. Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5. PLoS One 11:e0160875 (2016). Human . Read more (PubMed: 27504984) »
    • Tremblay N  et al. Spliceosome SNRNP200 Promotes Viral RNA Sensing and IRF3 Activation of Antiviral Response. PLoS Pathog 12:e1005772 (2016). WB . Read more (PubMed: 27454487) »

    See all 24 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Cell lysate - whole cell (liver)
    Specification liver
    Treatment 20 ug/ml poly(I:C)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Dec 26 2014

    Thank you for contacting us. The rabbit monoclonal isotyoe control ab128142 should be suitable for your purposes in Flow Cytometry as well. Do you also need any secondary antibodies for your experiment? We offer many fluorophore conjugated secondaries ...

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    Vielen Dank für Ihre Antwort.

    Ich möchte Ihnen einen kostenlosen Ersatz oder eine Gutschrift anbieten, da der Antikörper nicht so funktioniert wie wir diesen auf unserem Datenblatt beschreiben. Dazu benötige ich allerdings das ungefähre Best...

    Read More

    Vielen Dank für das Ausfüllen unseres Fragebogens.

    Ich habe nur noch zwei Fragen bezüglich Ihres Protokolls:

    1.Haben Sie einen Blot nur mit BSA geblockt und anschliessend den prim. Antikörper in TBST+ BSA inkubiert?
    Read More

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. We have not re...

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    Thank you for your call yesterday and for your patience while I have been in touch with the lab regarding ab76493. The phospho-specificity of the antibody was determined by Western blot and dot blot. The fluorescent detection may be more sensitive than...

    Read More