• Product nameAnti-IRF5 antibody [10T1]
    See all IRF5 primary antibodies
  • Description
    Mouse monoclonal [10T1] to IRF5
  • Specificityab33478 recognises IRF-5.
  • Tested applicationsSuitable for: ICC/IF, ELISA, IP, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human IRF-5 (176-240 aa, isoform D) purified from E. coli. NCBI Accession No.: NP_001092099 (formally NP_002191).

  • Positive control
    • Extracts of Ramos, THP-1 and A20 cells. HEK293



Our Abpromise guarantee covers the use of ab33478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interferon regulatory factor 5 antibody
    • Interferon regulatory factor 5 bone marrow variant antibody
    • IRF 5 antibody
    • IRF-5 antibody
    • Irf5 antibody
    • IRF5_HUMAN antibody
    • SLEB10 antibody
    see all

Anti-IRF5 antibody [10T1] images

  • Immunofluorescence/Immunocytochemistry analysis of HEK293 cells (105-106) stained with Mouse monoclonal [10T1] to IRF5 (ab33478 1/100-1/200).
  • All lanes : Anti-IRF5 antibody [10T1] (ab33478) at 1/1000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : THP-1 cell lysate
    Lane 3 : A20 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    goat anti-mouse secondary antibody conjugated to HRP
    developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 26 kDa,80 kDa. We are unsure as to the identity of these extra bands.
    100-200µg of whole cell lysates were used for western blotting.
  • Overlay histogram showing THP1 cells stained with ab33478 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33478, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab33478 at 1/50 dilution staining IRF5 in Human normal colon mucosa tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
  • ab33478 at 1/50 dilution staining IRF5 in Human colon adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The section was incubated with the primary antibody for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

References for Anti-IRF5 antibody [10T1] (ab33478)

This product has been referenced in:
  • O'Carroll C  et al. Identification of a unique hybrid macrophage-polarization state following recovery from lipopolysaccharide tolerance. J Immunol 192:427-36 (2014). Read more (PubMed: 24337373) »
  • Chionh YT  et al. Protease-activated receptor 1 suppresses Helicobacter pylori gastritis via the inhibition of macrophage cytokine secretion and interferon regulatory factor 5. Mucosal Immunol N/A:N/A (2014). Mouse . Read more (PubMed: 24866378) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Macrophages)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ventana Ultra CC1
Permeabilization No
Specification Macrophages
Blocking step OptiView H2O2 as blocking agent for 8 minute(s) · Concentration: 0.04% · Temperature: 36°C
Fixative Formaldehyde

Abcam user community

Verified customer

Submitted Jan 25 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2014

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - heat, Triton X-100
Blocking step Sniper (BioCare) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C

Ms. Colleen Karlo

Verified customer

Submitted Oct 04 2011

Thank you for your interest in our product, anti-IRF5 antibody(ab33478). I now have some more details which should answer your questions. 1. The anti-IRF5 Antibody (ab33478) was used at 1:1,000 dilution, and 30~50mg protein of cell lysates was used for...

Read More