- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-IRF5 antibody - ChIP Grade
See all IRF5 primary antibodies -
DescriptionRabbit polyclonal to IRF5 - ChIP Grade
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Tested applicationsSuitable for: CHIPseq, WBmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide conjugated to BSA derived from within residues 450 to the C-terminus of Human IRF5.
(Peptide available as ab22411.)
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Positive control
- This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) whole cell Liver (Mouse) Tissue Kidney (Mouse) Tissue Liver (Rat) Tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab21689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| CHIPseq | Use at an assay dependent concentration. PubMed: 25159141 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa). |
Application notes
Is unsuitable for IHC-P.
Target
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Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. -
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 615340 Cow
- Entrez Gene: 3663 Human
- Entrez Gene: 27056 Mouse
- Entrez Gene: 296953 Rat
- Omim: 607218 Human
- SwissProt: Q58DJ0 Cow
- SwissProt: Q13568 Human
- SwissProt: P56477 Mouse
see all -
Alternative names
- Interferon regulatory factor 5 antibody
- Interferon regulatory factor 5 bone marrow variant antibody
- IRF 5 antibody
see all
Anti-IRF5 antibody - ChIP Grade images
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CHIPseq - Anti-IRF5 antibody - ChIP Grade (ab21689)Image from Saliba DG et al., Cell Rep. 2014;8(5):1308-17. Fig 5(A).; doi: 10.1016/j.celrep.2014.07.034.GM-BMDMs from RelAFl/Fl mice were infected with either Cre (black bars) or Empty (white bars) adenovirus and stimulated with LPS (100ng/ml) for 2 hr or left unstimulated and used in ChIP analysis for IRF5 recruitment on Il-6 and Il-1a loci. Nuclear lysates were immunoprecipitated with 10µg of ab21689. Data indicate mean percent input relative to genomic DNA ± SD of a representative experiment.
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Western blot - IRF5 antibody (ab21689)
Predicted band size : 58 kDa -
Western blot - IRF5 antibody (ab21689)All lanes : Anti-IRF5 antibody - ChIP Grade (ab21689) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Protocols
References for Anti-IRF5 antibody - ChIP Grade (ab21689)
This product has been referenced in:
- Ban T et al. Lyn Kinase Suppresses the Transcriptional Activity of IRF5 in the TLR-MyD88 Pathway to Restrain the Development of Autoimmunity. Immunity 45:319-32 (2016). Mouse . Read more (PubMed: 27521268) »
- Masuda T et al. Transcription factor IRF5 drives P2X4R+-reactive microglia gating neuropathic pain. Nat Commun 5:3771 (2014). WB, ChIP . Read more (PubMed: 24818655) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"