Overview

  • Product nameAnti-IRF5 antibody - ChIP Grade
    See all IRF5 primary antibodies
  • Description
    Rabbit polyclonal to IRF5 - ChIP Grade
  • Tested applicationsSuitable for: CHIPseq, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide conjugated to BSA derived from within residues 450 to the C-terminus of Human IRF5.

    (Peptide available as ab22411.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) whole cell Liver (Mouse) Tissue Kidney (Mouse) Tissue Liver (Rat) Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab21689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
CHIPseq Use at an assay dependent concentration. PubMed: 25159141
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
  • Application notesIs unsuitable for IHC-P.
  • Target

    • Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
    • Sequence similaritiesBelongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Interferon regulatory factor 5 antibody
      • Interferon regulatory factor 5 bone marrow variant antibody
      • IRF 5 antibody
      • IRF-5 antibody
      • Irf5 antibody
      • IRF5_HUMAN antibody
      • SLEB10 antibody
      see all

    Anti-IRF5 antibody - ChIP Grade images

    • GM-BMDMs from RelAFl/Fl mice were infected with either Cre (black bars) or Empty (white bars) adenovirus and stimulated with LPS (100ng/ml) for 2 hr or left unstimulated and used in ChIP analysis for IRF5 recruitment on Il-6 and Il-1a loci. Nuclear lysates were immunoprecipitated with 10µg of ab21689. Data indicate mean percent input relative to genomic DNA ± SD of a representative experiment.



    • Predicted band size : 58 kDa
    • All lanes : Anti-IRF5 antibody - ChIP Grade (ab21689) at 1 µg/ml

      Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
      Lane 2 : Kidney (Mouse) Tissue Lysate
      Lane 3 : Liver (Rat) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 58 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)

    References for Anti-IRF5 antibody - ChIP Grade (ab21689)

    This product has been referenced in:
    • Masuda T  et al. Transcription factor IRF5 drives P2X4R+-reactive microglia gating neuropathic pain. Nat Commun 5:3771 (2014). WB, ChIP . Read more (PubMed: 24818655) »
    • Saliba DG  et al. IRF5:RelA Interaction Targets Inflammatory Genes in Macrophages. Cell Rep 8:1308-17 (2014). CHIPseq ; Mouse . Read more (PubMed: 25159141) »

    See all 8 Publications for this product

    Product Wall

    Unfortunately, this antibody has not tested with IP. Instead, ab33478 works with mouse on IP.

    Thank you for your enquiry. I believe I just spoke with you this afternoon regarding this antibody. Thank you for sending the paper, I can add it to our general/background references for this antibody. I discussed the matter with one of my colle...

    Read More
    Abreviews
    Application Western blot
    Sample Human Cell lysate - nuclear (human monocytes)
    Loading amount 20 µg
    Specification human monocytes
    Treatment LPS 100ug/ml for 3h
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5
    Username

    Dr. Haiyan Chen

    Verified customer

    Submitted Jan 03 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"