Anti-IRF5 antibody (ab21689)
Key features and details
- Rabbit polyclonal to IRF5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-IRF5 antibody
See all IRF5 primary antibodies -
Description
Rabbit polyclonal to IRF5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Human IRF5 aa 450 to the C-terminus (C terminal) conjugated to bovine serum albumin.
(Peptide available asab22411) -
Positive control
- This antibody gave a positive signal in the following lysates: HeLa (Human epithelial carcinoma cell line) whole cell Liver (Mouse) Tissue Kidney (Mouse) Tissue Liver (Rat) Tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa). |
Target
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Involvement in disease
Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. -
Sequence similarities
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 615340 Cow
- Entrez Gene: 3663 Human
- Entrez Gene: 27056 Mouse
- Entrez Gene: 296953 Rat
- Omim: 607218 Human
- SwissProt: Q58DJ0 Cow
- SwissProt: Q13568 Human
- SwissProt: P56477 Mouse
see all -
Alternative names
- Interferon regulatory factor 5 antibody
- Interferon regulatory factor 5 bone marrow variant antibody
- IRF 5 antibody
see all
Images
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All lanes : Anti-IRF5 antibody (ab21689) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 20 µg
Lane 2 : Mouse Liver Tissue Lysate at 10 µg
Lane 3 : Mouse Kidney Tissue Lysate at 10 µg
Lane 4 : Rat Liver Tissue Lysate at 10 µg
Secondary
All lanes : Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab21689 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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All lanes : Anti-IRF5 antibody (ab21689) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate with Human IRF5 peptide (ab22411) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to IgG (700) at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-IRF5 antibody (ab21689) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab21689 has been referenced in 24 publications.
- Allouch A et al. CDKN1A is a target for phagocytosis-mediated cellular immunotherapy in acute leukemia. Nat Commun 13:6739 (2022). PubMed: 36347876
- Kawase W et al. Irf5 siRNA-loaded biodegradable lipid nanoparticles ameliorate concanavalin A-induced liver injury. Mol Ther Nucleic Acids 25:708-715 (2021). PubMed: 34589288
- Yousif AS et al. The persistence of interleukin-6 is regulated by a blood buffer system derived from dendritic cells. Immunity 54:235-246.e5 (2021). PubMed: 33357409
- Pradhan P et al. TRAF6-IRF5 kinetics, TRIF, and biophysical factors drive synergistic innate responses to particle-mediated MPLA-CpG co-presentation. Sci Adv 7:N/A (2021). PubMed: 33523878
- Ban T et al. Genetic and chemical inhibition of IRF5 suppresses pre-existing mouse lupus-like disease. Nat Commun 12:4379 (2021). PubMed: 34282144