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Synthetic peptide: QGPWPMHPAGMQ, corresponding to C terminal amino acids 493-504 of Human IRF5.
Principal Names – IRF5; interferon regulatory factor 5 Official Gene Symbol - IRF5 GenBank Accession Number – NP_002191; NP_116032 LocusLink ID - 3663 (human) Gene Ontology terms - RNA polymerase III transcription factor; cell growth and/or maintenance
Our Abpromise guarantee covers the use of ab2932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent dilution. PubMed: 19028697|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).Can be blocked with Human IRF5 peptide (ab22939).|
|IP||Use at an assay dependent dilution. PubMed: 19028697|
p53± cells were treated over a time course. The cells were subjected to IP and ChIP analysis using the indicated antibodies and primers. Input is shown in the top four panels; PCR results are shown in the bottom panels. A single panel is shown as representative of amplification from all primer sets after IP with IgG control antibodies for IRF-5.
HEK293 (ab7902) overexpressing human IRF5 and probed with ab2932 at 1µg/ml (mock transfection in lane 2).
IHC image of ab2932 staining in human normal cervix (ab29226) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2932, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.