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Anti-IRF5 antibody - ChIP Grade (ab2932)

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Overview

Product name

Anti-IRF5 antibody - ChIP Grade
See all IRF5 products (7) ...

Description

Goat polyclonal to IRF5 - ChIP Grade

Specificity

This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1).

Tested applications

IHC-P, ChIP, WB, IPmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

Synthetic peptide: QGPWPMHPAGMQ, corresponding to C terminal amino acids 493-504 of Human IRF5.

C-QGPWPMHPAGMQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

A549 lysate

General notes

Principal Names – IRF5; interferon regulatory factor 5 Official Gene Symbol - IRF5 GenBank Accession Number – NP_002191; NP_116032 LocusLink ID - 3663 (human) Gene Ontology terms - RNA polymerase III transcription factor; cell growth and/or maintenance

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline

Concentration

Concentration information loading...

Purity

IgG fraction

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> Helix-Turn-Helix
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol III Transcription
Immunology >> Innate Immunity >> Cytokines >> Interferons

  • Western blot - IRF5 antibody (ab2932)Western blot - IRF5 antibody (ab2932) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-IRF5 antibody - ChIP Grade(ab2932)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-IRF5 antibody - ChIP Grade(ab2932) image (enlarge)

  • Western blot - Anti-IRF5 antibody - ChIP Grade (ab2932)Western blot - Anti-IRF5 antibody - ChIP Grade (ab2932) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ShowHide

    ChIP

     ChIP: Use at an assay de...Read more →

    ChIP: Use at an assay dependent dilution. PubMed: 19028697

  • ShowHide2 Images

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution. PubMed: 19028697

Target

Involvement in disease

Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.

Sequence similarities

Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13568 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Interferon regulatory factor 5 antibody
  • IRF 5 antibody
  • IRF-5 antibody
  • Irf5 antibody
  • IRF5_HUMAN antibody
  • SLEB10 antibody
see all

Database links

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Anti-IRF5 antibody - ChIP Grade images:

  Western blot - IRF5 antibody (ab2932)

Western blot - IRF5 antibody (ab2932)



Predicted band size : 58 kDa


ab2932 staining (2µg/ml) of A549 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-IRF5 antibody - ChIP Grade(ab2932)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-IRF5 antibody - ChIP Grade(ab2932)

IHC image of ab2932 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2932, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  Western blot - Anti-IRF5 antibody - ChIP Grade (ab2932)

Western blot - Anti-IRF5 antibody - ChIP Grade (ab2932)



Predicted band size : 58 kDa


HEK293 overexpressing human IRF5 and probed with ab2932 at 1µg/ml (mock transfection in lane 2).

References for Anti-IRF5 antibody - ChIP Grade (ab2932)

This product has been referenced in:

  • Krausgruber T  et al. IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses. Nat Immunol 12:231-8 (2011). WB; Human.Read more (PubMed: 21240265) »
  • Krausgruber T  et al. IRF5 is required for late-phase TNF secretion by human dendritic cells. Blood 115:4421-30 (2010). WB, ChIP, IP; Human.Read more (PubMed: 20237317) »

See all 7 publications for this product

Publishing research using ab2932? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"