Overview

  • Product nameAnti-IRF5 antibody [EPR6094]
    See all IRF5 primary antibodies
  • Description
    Rabbit monoclonal [EPR6094] to IRF5
  • Tested applicationsSuitable for: IHC-Fr, WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human IRF5 (Q13568).

  • Positive control
    • U937, Ramos and THP-1 cell lysates, U937 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-IRF5 antibody (Alexa Fluor® 488) [EPR6094] (ab193245)

    Anti-IRF5 antibody (Alexa Fluor® 647) [EPR6094] (ab192983)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).Can be blocked with IRF5 peptide (ab174256).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notesIs unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Involvement in diseaseGenetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
    • Sequence similaritiesBelongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Interferon regulatory factor 5 antibody
      • Interferon regulatory factor 5 bone marrow variant antibody
      • IRF 5 antibody
      • IRF-5 antibody
      • Irf5 antibody
      • IRF5_HUMAN antibody
      • SLEB10 antibody
      see all

    Anti-IRF5 antibody [EPR6094] images

    • All lanes : Anti-IRF5 antibody [EPR6094] (ab124792) at 1/1000 dilution

      Lane 1 : U937 cell lysates
      Lane 2 : Ramos cell lysates
      Lane 3 : THP-1 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • Flow cytometric analysis of permeabilized U937 cells using ab124792 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Flow cytometry analysis of Plasmacytoid dendritic cells, staining IRF5 with ab124792.

      Cells were fixed in paraformaldehyde and fixed in saponin. The sample was incubated with the primary antibody (1/100 in 2% human serum + 0.5 mM EDTA) for 20 minutes at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.

      Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

      See Abreview

    References for Anti-IRF5 antibody [EPR6094] (ab124792)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Human Cell (pDC)
    Specification pDC
    Preparation Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS
    Fixation Paraformaldehyde
    Permeabilization Yes - saponin
    Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
    Username

    Abcam user community

    Verified customer

    Submitted Oct 04 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (tonsil)
    Specification tonsil
    Fixative Acetone
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"