Anti-IRF6 antibody [2A12] (ab123880)

Overview

  • Product nameAnti-IRF6 antibody [2A12]
    See all IRF6 primary antibodies
  • Description
    Mouse monoclonal [2A12] to IRF6
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human IRF6 protein (NP_006138) produced in HEK293 cell.

  • Positive control
    • Purchase matching WB positive control:Recombinant Human IRF6 protein
    • WB: HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA IHC-P: adenocarcinoma of Human colon tissue, Human kidney tissue, carcinoma of Human lung tissue, adenocarcinoma of Human endometrium tissue ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY IRF6 Flow Cyt: HEK293T cells transfected with pCMV6-ENTRY IRF6 overexpress plasmid
  • General notesDilute in PBS (pH 7.3) before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab123880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 53 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionProbable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
  • Tissue specificityExpressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
  • Involvement in diseaseDefects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
  • Cellular localizationNucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interferon regulatory factor 6 antibody
    • IRF 6 antibody
    • IRF-6 antibody
    • IRF6 antibody
    • IRF6_HUMAN antibody
    • LPS antibody
    • OFC 6 antibody
    • OFC6 antibody
    • OTTHUMP00000034677 antibody
    • OTTHUMP00000034678 antibody
    • PIT antibody
    • PPS antibody
    • PPS1 antibody
    • VWS antibody
    • VWS1 antibody
    see all

Anti-IRF6 antibody [2A12] images

  • All lanes : Anti-IRF6 antibody [2A12] (ab123880) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201579)
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human colon tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded carcinoma of Human lung tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
  • ab123880, at a 1/100 dilution, staining IRF6 in COS7 cells transiently transfected using pCMV6-ENTRY IRF6, by Immunofluorescence.
  • ab123880, at a 1/100 dilution, staining IRF6 in HEK293T cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue), by Flow Cytometry.

References for Anti-IRF6 antibody [2A12] (ab123880)

ab123880 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Granular Cell Layer)
Specification Granular Cell Layer
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum, plus monovalent Fab Fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 18 2012

Thank you for your phone call.

I have corrected the datasheet for ab10925. Thank you so much for pointing out the typos!

DISCOUNT CODE: xxx for ab115220
DISCOUNT CODE: xxx for ab123880
Expiration date: xxx

I am...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"