Our Abpromise guarantee covers the use of ab58915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.


  • FunctionProbable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
  • Tissue specificityExpressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
  • Involvement in diseaseDefects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • Sequence similaritiesBelongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
  • Cellular localizationNucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
  • Information by UniProt
  • Database links
  • Alternative names
    • Interferon regulatory factor 6 antibody
    • IRF 6 antibody
    • IRF-6 antibody
    • IRF6 antibody
    • IRF6_HUMAN antibody
    • LPS antibody
    • OFC 6 antibody
    • OFC6 antibody
    • OTTHUMP00000034677 antibody
    • OTTHUMP00000034678 antibody
    • PIT antibody
    • PPS antibody
    • PPS1 antibody
    • VWS antibody
    • VWS1 antibody
    see all

Anti-IRF6 antibody images

  • Anti-IRF6 antibody (ab58915) at 2.5 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.

References for Anti-IRF6 antibody (ab58915)

This product has been referenced in:
  • Zengin T  et al. IRF6 Is Involved in the Regulation of Cell Proliferation and Transformation in MCF10A Cells Downstream of Notch Signaling. PLoS One 10:e0132757 (2015). WB ; Human . Read more (PubMed: 26161746) »
  • Pan Y  et al. IRF6 polymorphisms are associated with nonsyndromic orofacial clefts in a Chinese Han population. Am J Med Genet A 152A:2505-11 (2010). WB ; Human . Read more (PubMed: 20799332) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Dog Tissue lysate - whole (kidney and spleen)
Gel Running Conditions Reduced Denaturing (4-20% polyacrylamide)
Loading amount 20 µg
Specification kidney and spleen
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Fibroblasts)
Loading amount 35 µg
Specification Fibroblasts
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel, MOPS Buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Lukas Rambousek

Verified customer

Submitted Dec 14 2010