Overview

  • Product name
    Anti-IRF7 antibody [3D9]
    See all IRF7 primary antibodies
  • Description
    Mouse monoclonal [3D9] to IRF7
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to N terminal amino acids 1-150 of Human IRF7 purified from E.coli.

  • Positive control
    • Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
  • Tissue specificity
    Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
    TRAF6-mediated ubiquitination is required for IRF7 activation.
  • Cellular localization
    Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • IMD39 antibody
    • Interferon regulatory factor 7 antibody
    • Interferon regulatory factor 7H antibody
    • IRF 7 antibody
    • IRF 7A antibody
    • IRF 7H antibody
    • IRF-7 antibody
    • IRF7 antibody
    • IRF7_HUMAN antibody
    • IRF7A antibody
    • IRF7B antibody
    • IRF7C antibody
    • IRF7H antibody
    see all

Images

  • Anti-IRF7 antibody [3D9] (ab70069) at 1/1000 dilution + Jurkat cell lysates at 40 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References

ab70069 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70069.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up