The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
Predominantly in lymphoid tissues.
Belongs to the IRF family. Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
Detection of IRF8 in Immunoprecipitates of RAW 264.7 whole cell lysate (0.5 mg for IP, 20% of IP loaded) using ab168808 at 6 µg/mg lysate for IP. For WB detection an alternative anti-IRF8 antibody was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.