Overview

  • Product name
  • Description
    Rabbit polyclonal to IRF8
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human IRF8.

  • Positive control
    • This antibody gave a positive signal in Human lymph node tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab93718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

Target

  • Function
    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • Tissue specificity
    Predominantly in lymphoid tissues.
  • Sequence similarities
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Post-translational
    modifications
    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

Images

  • All lanes : Anti-IRF8 antibody (ab93718) at 1 µg/ml

    Lane 1 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871) with immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa
    Additional bands at : 26 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

References

ab93718 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93718.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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