Overview

  • Product nameAnti-IRG1 antibody
    See all IRG1 primary antibodies
  • Description
    Rabbit polyclonal to IRG1
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: FPAHLSTHWV ADAAASVRKH LVAERALLPT DYIKRIVLRI PNVQYVNRPF PVSEHEARHS FQYVACAMLL DGGITVPSFH ECQINRPQVR ELLSKVELEY, corresponding to internal sequence amino acids 274-373 of Human IRG1.

  • Positive control
    • RT 4 and U 251 MG lysate; Human rectum tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

Anti-IRG1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122624 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122624 at 1/200 dilution staining IRG1 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
  • All lanes : Anti-IRG1 antibody (ab122624) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References for Anti-IRG1 antibody (ab122624)

This product has been referenced in:
  • Li Y  et al. Immune responsive gene 1 (IRG1) promotes endotoxin tolerance by increasing A20 expression in macrophages through reactive oxygen species. J Biol Chem 288:16225-34 (2013). WB . Read more (PubMed: 23609450) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest our Anti-IRG1 antibody (ab122624) .

To our knowledge, the product has not been tested in mouse. However the comparison of the immunogen sequence results in an identity of 82% between the human and mouse se...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"