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antigen sequence: FPAHLSTHWV ADAAASVRKH LVAERALLPT DYIKRIVLRI PNVQYVNRPF PVSEHEARHS FQYVACAMLL DGGITVPSFH ECQINRPQVR ELLSKVELEY, corresponding to internal sequence amino acids 274-373 of Human IRG1.
Our Abpromise guarantee covers the use of ab122624 in the following tested applications.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment