The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Block using 3% milk.
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
Involvement in disease
Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
Belongs to the interferon-inducible GTPase family.
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
Abcam recommends using milk as the blocking agent. There are two isoforms of Mouse IRGM protein. Isoform 1 and 2 are 47- and 45-kDa respectively (SwissProt). This would explain the observation of a doublet on the Western Blot. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.