Overview

  • Product name
  • Description
    Rabbit polyclonal to IRGM
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 17 amino acid peptide near the amino terminus of human IRGM.

  • Positive control
    • WB: Rat brain lysate. IHC-P: Human thymus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab69494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21102437
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21-46 kDa). please refer to databases and the literature for the expected molecular weights of species orthologues.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
  • Tissue specificity
    Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
  • Involvement in disease
    Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similarities
    Belongs to the interferon-inducible GTPase family.
  • Cellular localization
    Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFI1 antibody
    • Iigp3 antibody
    • Iipg3 antibody
    • Immunity related GTPase family M protein 1 antibody
    • Immunity related GTPase family, M antibody
    • Immunity-related GTPase family M protein 1 antibody
    • Immunity-related GTPase family M protein antibody
    • Interferon inducible protein 1 antibody
    • Interferon-inducible protein 1 antibody
    • Irgm antibody
    • IRGM_HUMAN antibody
    • IRGM1 antibody
    • LPS-stimulated RAW 264.7 macrophage protein 47 homolog antibody
    • LRG 47 antibody
    • LRG-47 antibody
    • LRG-47-like protein antibody
    • LRG47 antibody
    • MGC149263 antibody
    • MGC149264 antibody
    see all

Images

  • Paraffin-embedded human thymus tissue stained for IRGM using ab69494 at 5 µg/ml in immunohistochemical analysis.

  • IHC image of ab69494 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69494, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Hansen MD  et al. Hepatitis C virus triggers Golgi fragmentation and autophagy through the immunity-related GTPase M. Proc Natl Acad Sci U S A 114:E3462-E3471 (2017). Read more (PubMed: 28389568) »
  • Springer HM  et al. Irgm1 (LRG-47), a regulator of cell-autonomous immunity, does not localize to mycobacterial or listerial phagosomes in IFN-?-induced mouse cells. J Immunol 191:1765-74 (2013). WB . Read more (PubMed: 23842753) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (HEK293T, Hela)
Specification
HEK293T, Hela
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2014

Vielen Dank für Ihre Anfrage.

Der Austausch des ab69495 Anti-IRGM antibody gegen den ab65290 ist ueberhaupt kein Problem.

Ich eine kostenlose Ersatzlieferung fuer Sie in Auftrag gegeben. Sie hat die Referenznummer XXXXXX und ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount
150000 cells
Specification
Mouse Embryonic Fibroblasts
Treatment
200U/ml interferon-gamma 24h
Gel Running Conditions
Reduced Denaturing (12% PA-SAS gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 26 2012

DISCOUNT CODE: xx
Ablaufdatum: dd mm 2012

Es freut mich zu hoeren, dass Sie unser Angebot unseren Antikoerper ab69494 mit den verschiedenen Konstrukten zu benutzen (und dann eine Abbildung einzusenden), annehmen moechten . Wie am Telefo...

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Vielen Dank für Ihren Anruf. Leider kann ich die genaue Sequenz des Immunogens nicht an Sie weitergeben, weil es sich hierbei um ein Betriebsgeheimnis handelt. Allerdings darf ich Ihnen mitteilen, dass das Immunogen zwischen den Aminosäuren 25 und 75 ...

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