Overview

  • Product nameAnti-IRGM antibody
    See all IRGM primary antibodies
  • Description
    Rabbit polyclonal to IRGM
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IRGM.

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Small Intestine; Colon.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 20 kDa).

Target

  • FunctionPutative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
  • Tissue specificityWidely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
  • Involvement in diseaseDefects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
  • Sequence similaritiesBelongs to the interferon-inducible GTPase family.
  • Cellular localizationGolgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • IFI1 antibody
    • Iigp3 antibody
    • Iipg3 antibody
    • Immunity related GTPase family M protein 1 antibody
    • Immunity related GTPase family, M antibody
    • Immunity-related GTPase family M protein 1 antibody
    • Immunity-related GTPase family M protein antibody
    • Interferon inducible protein 1 antibody
    • Interferon-inducible protein 1 antibody
    • Irgm antibody
    • IRGM_HUMAN antibody
    • IRGM1 antibody
    • LPS-stimulated RAW 264.7 macrophage protein 47 homolog antibody
    • LRG 47 antibody
    • LRG-47 antibody
    • LRG-47-like protein antibody
    • LRG47 antibody
    • MGC149263 antibody
    • MGC149264 antibody
    see all

Anti-IRGM antibody images

  • All lanes : Anti-IRGM antibody (ab93901) at 1 µg/ml

    Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References for Anti-IRGM antibody (ab93901)

This product has been referenced in:
  • Grégoire IP  et al. IRGM Is a Common Target of RNA Viruses that Subvert the Autophagy Network. PLoS Pathog 7:e1002422 (2011). WB . Read more (PubMed: 22174682) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293)
Gel Running Conditions Reduced Denaturing
Loading amount 3.5 µg
Specification HEK 293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Ryan Russell

Verified customer

Submitted Sep 02 2016

Voici le protocole utilisé par le laboratoire : - 10µg de lysat par puit (under reducing conditions), - transfert sur membrane nitrocellulose (1 heure, 90V), - blocage avec 5% de BSA (1 heure, température ambiante), - incubation du primaire...

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Merci pour toutes ces précisions. Nous sommes désolés que cet anticorps ne fonctionne pas avec la protéine endogène comme décrit sur la fiche technique. Nous disposons malheureusement que d'un seul lot de ab93901. Je peux vous faire parv...

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Je suis désolé d'apprendre que ab93901 ne vous donne pas de bons résultats pour la détection de IRGM endogène. Le laboratoire et moi-même aimerions avoir plus d'informations sur le protocole que vous avez utilisé avec cet anticorps. Veuillez tr...

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Thank you for contacting us with your question. I will be happy to check with the lab and see if we can add the blocking peptide for ab69495 to the catalog. If you would like, you can also send an image of your blot and some information about yo...

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