The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).
Use at an assay dependent concentration. PubMed: 22992950
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
Belongs to the TALE/IRO homeobox family. Contains 1 homeobox DNA-binding domain.