Recombinant
RabMAb

Anti-Iroquois homeobox protein 3 antibody [EPR12124] (ab174307)

Overview

  • Product name
    Anti-Iroquois homeobox protein 3 antibody [EPR12124]
    See all Iroquois homeobox protein 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12124] to Iroquois homeobox protein 3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Iroquois homeobox protein 3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P78415

  • Positive control
    • Hela, Jurkat, A431 and K562 cell lysates; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12124
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells.
    • Sequence similarities
      Belongs to the TALE/IRO homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI894186 antibody
      • Homeodomain protein IRXB1 antibody
      • Iroquois class homeodomain protein IRX 1 antibody
      • Iroquois homeobox 3 antibody
      • Iroquois homeobox protein 3 antibody
      • Iroquois-class homeodomain protein irx-3 antibody
      • IRX 1 antibody
      • irx3 antibody
      • IRX3_HUMAN antibody
      • IRXB1 antibody
      see all

    Images

    • All lanes : Anti-Iroquois homeobox protein 3 antibody [EPR12124] (ab174307) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Flow cytometric analysis of permeabilized K562 cells labeling Iroquois homeobox protein 3 with ab174307 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Singh B  et al. Important Role of FTO in the Survival of Rare Panresistant Triple-Negative Inflammatory Breast Cancer Cells Facing a Severe Metabolic Challenge. PLoS One 11:e0159072 (2016). WB ; Human . Read more (PubMed: 27390851) »
    • Landgraf K  et al. FTO Obesity Risk Variants Are Linked to Adipocyte IRX3 Expression and BMI of Children - Relevance of FTO Variants to Defend Body Weight in Lean Children? PLoS One 11:e0161739 (2016). WB . Read more (PubMed: 27560134) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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