Overview

  • Product name
    Anti-IRS1 (phospho S1101) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S1101)
  • Specificity
    This antibody detects endogenous levels of IRS1 only when phosphorylated at serine 1101.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 1101 (H-S-SP-E-T).

  • Positive control
    • Human colon carcinoma tissue, extracts from COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab55343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notes
    ELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight for Green monkey: 133 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • Involvement in disease
      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • Sequence similarities
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Images

    • This image shows human colon carcinoma tissue stained with ab55343 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising phosphopeptide.
    • All lanes : Anti-IRS1 (phospho S1101) antibody (ab55343) at 1/500 dilution

      Lane 1 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins.
      Lane 2 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins and with the immunising phosphopeptide.


      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    References

    This product has been referenced in:
    • Stuart CA  et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2: (2014). WB . Read more (PubMed: 25472611) »

    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab55343.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up