The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight for Green monkey: 133 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in disease
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.
This image shows human colon carcinoma tissue stained with ab55343 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising phosphopeptide.
Western blot - IRS1 (phospho S1101) antibody (ab55343)
All lanes : Anti-IRS1 (phospho S1101) antibody (ab55343) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins. Lane 2 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins and with the immunising phosphopeptide.
Predicted band size : 133 kDa Observed band size : 133 kDa