Anti-IRS1 (phospho S1101) antibody (ab55343)

Overview

  • Product nameAnti-IRS1 (phospho S1101) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S1101)
  • SpecificityThis antibody detects endogenous levels of IRS1 only when phosphorylated at serine 1101.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human IRS1 around the phosphorylation site of serine 1101 (H-S-SP-E-T).

  • Positive control
    • Human colon carcinoma tissue, extracts from COS7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab55343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 133 kDa (predicted molecular weight for Green monkey: 133 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • Sequence similaritiesContains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Anti-IRS1 (phospho S1101) antibody images

    • This image shows human colon carcinoma tissue stained with ab55343 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising phosphopeptide.
    • All lanes : Anti-IRS1 (phospho S1101) antibody (ab55343) at 1/500 dilution

      Lane 1 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins.
      Lane 2 : Extracts from COS7 cells treated with Calyculin A at 50ng/ml for 30 mins and with the immunising phosphopeptide.


      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    References for Anti-IRS1 (phospho S1101) antibody (ab55343)

    ab55343 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab55343.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"