Overview

  • Product name
    Anti-IRS1 (phospho S307) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S307)
  • Specificity
    Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab1194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).

Target

  • Function
    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease
    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.

    Lysates/proteins at 25 µg per lane.

    Secondary
    IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 131 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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