Overview

  • Product nameAnti-IRS1 (phospho S312) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S312)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig
  • Immunogen

    Synthetic peptide (Human) derived from a region of IRS 1 that contains serine 312.

  • Positive control
    • Phorbol ester stimulated (TPA) Chinese Hamster Ovary cell line expressing human insulin receptor (CHO-T) and transiently transfected with a plasmid encoding human IRS 1.

Properties

Applications

Our Abpromise guarantee covers the use of ab4865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: We recommend a starting dilution of 1/1000. Predicted molecular weight: 165 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • Sequence similaritiesContains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Anti-IRS1 (phospho S312) antibody images



    • Predicted band size : 165 kDa


      Peptide Competition and Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with wild-type human IRS 1 and treated with TPA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were treated with lambda phosphatase (1) or left untreated (2-6), blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with 0.50 µg/mL ab4865 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4865 blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

    References for Anti-IRS1 (phospho S312) antibody (ab4865)

    This product has been referenced in:
    • Dávila D & Torres-Aleman I Neuronal Death by Oxidative Stress Involves Activation of FOXO3 through a Two-Arm Pathway that Activates Stress Kinases and Attenuates Insulin-like Growth Factor I Signalling. Mol Biol Cell : (2008). WB ; Rat . Read more (PubMed: 18287535) »

    See 1 Publication for this product

    Product Wall

    Ab3688 and Ab4865 are two different antibodies, we get them from two different sources. Both are in stock. Both have only been tested for cross-reactivity with human. Ab3688 is specific for human IRS 1 phosphorylated at the position of Serine 312...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"