Overview

  • Product nameAnti-IRS1 (phospho S312) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S312)
  • Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide corresponding to the epitope ATSPA, with a single phosphorylation site, Ser 312, from human IRS1.

  • Positive control
    • Insulin stimulated CHO cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab66154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 132 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similaritiesContains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho S312) antibody images

  • 1µg peptides were blotted onto a nitrocellulose membrane followed by ab66154 at 1/1000 dilution. Peptides: A: IRS1(pS312) B: IRS1(non phosphorylated) C: Non-related Phosphopeptide

References for Anti-IRS1 (phospho S312) antibody (ab66154)

This product has been referenced in:
  • Fabre O  et al. Defects in TLR3 expression and RNase L activation lead to decreased MnSOD expression and insulin resistance in muscle cells of obese people. Cell Death Dis 5:e1136 (2014). WB ; Human . Read more (PubMed: 24651439) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"