Anti-IRS1 (phospho S522) antibody (ab65745)

Overview

  • Product nameAnti-IRS1 (phospho S522) antibody
    See all IRS1 primary antibodies
  • Description
    Sheep polyclonal to IRS1 (phospho S522)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide: CFRKRTHSPAGT conjugated to KLH, corresponding to amino acids 521-530 of Human IRS1

  • Positive control
    • Over-expressed IRS1 protein in extracts from CHO.IR.IRS1 with or without insulin treatment.

Properties

Applications

Our Abpromise guarantee covers the use of ab65745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 132 kDa.

Target

  • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similaritiesContains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

References for Anti-IRS1 (phospho S522) antibody (ab65745)

ab65745 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - other (Skeletal muscle, human)
Loading amount 100 µg
Specification Skeletal muscle, human
Gel Running Conditions Reduced Denaturing (5 %)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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Submitted Aug 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"