Anti-IRS1 (phospho S616) antibody (ab58479)

Overview

  • Product name
    Anti-IRS1 (phospho S616) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S616)
  • Specificity
    ab58479 detects endogenous levels of IRS1 only when phosphorylated at serine 612.
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IRS1.

  • Positive control
    • Human breast carcinoma tissue; HUVEC lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

Target

  • Function
    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in disease
    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similarities
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Images

  • All lanes : Anti-IRS1 (phospho S616) antibody (ab58479) at 1/500 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate with Phospho peptide


    Predicted band size : 131 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

References

ab58479 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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