Anti-IRS1 (phospho S616) antibody (ab58479)

Overview

  • Product nameAnti-IRS1 (phospho S616) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho S616)
  • Specificityab58479 detects endogenous levels of IRS1 only when phosphorylated at serine 612.
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human IRS1.

  • Positive control
    • Human breast carcinoma tissue; HUVEC lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.
WB 1/500 - 1/1000. Predicted molecular weight: 131 kDa.

Target

  • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similaritiesContains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho S616) antibody images

  • All lanes : Anti-IRS1 (phospho S616) antibody (ab58479) at 1/500 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cells) treated with insulin 0.01U/mL30' cell lysate with Phospho peptide


    Predicted band size : 131 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab58479 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

References for Anti-IRS1 (phospho S616) antibody (ab58479)

ab58479 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab58479.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"