• Product nameAnti-IRS1 (phospho Y612) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho Y612)
  • SpecificityCross-reactivty with IRS2 has not been tested. Immunogen for ab66153 has 75% homology with corresponding IRS2 sequence.
  • Tested applicationsSuitable for: IHC-P, WB, IP, Indirect ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to the epitope DGYMP with a single phosphorylation site, Tyr612, from human IRS1.

  • Positive control
    • Insulin stimulated CHO cell lysate.



Our Abpromise guarantee covers the use of ab66153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 165 kDa (predicted molecular weight: 132 kDa).
IP Use a concentration of 2 - 5 µg/ml.
Indirect ELISA Use a concentration of 0.01 - 0.1 µg/ml.


  • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similaritiesContains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho Y612) antibody images

  • Immunoprecipitation analysis of insulin stimulated CHO cells precipitating IRS1 (phospho Y912) with ab66153. Western blot was performed with ab66153 at 1:500 (Lane 1). Lane 2 is a negative control.

  • 1µg of peptide was placed on a nitrocellulose membrane.

    Peptides: A: IRS1(pY612) B: IRS1 (non phosphorylated) C: Non-related Phosphopeptide.

    Lane 1: ab66153 at 1/1000. Lane 2: Rabbit anti-non-pY612 IRS1 at 1/1000.

References for Anti-IRS1 (phospho Y612) antibody (ab66153)

This product has been referenced in:
  • Pan T  et al. Cytohesins/ARNO: the function in colorectal cancer cells. PLoS One 9:e90997 (2014). Read more (PubMed: 24618737) »
  • Ujvari D  et al. Lifestyle intervention up-regulates gene and protein levels of molecules involved in insulin signaling in the endometrium of overweight/obese women with polycystic ovary syndrome. Hum Reprod N/A:N/A (2014). Read more (PubMed: 24842895) »

See all 5 Publications for this product

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