Recombinant
RabMAb

Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)

Overview

  • Product name
    Anti-IRS1 (phospho Y632) antibody [EPR259(2)]
    See all IRS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR259(2)] to IRS1 (phospho Y632)
  • Specificity
    ab109543 only detects IRS1 phosphorylated at Tyrosine 632.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 632 of Human IRS1.

  • Positive control
    • 293T cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • Involvement in disease
      Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • Sequence similarities
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Images

    • All lanes : Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543) at 1/1000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate treated with Alkaline Phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 132 kDa

    References

    This product has been referenced in:
    • Song Q  et al. Concomitant inhibition of receptor tyrosine kinases and downstream AKT synergistically inhibited growth of KRAS/BRAF mutant colorectal cancer cells. Oncotarget 8:5003-5015 (2017). Read more (PubMed: 28002807) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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