Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)

Overview

  • Product nameAnti-IRS1 (phospho Y632) antibody [EPR259(2)]
    See all IRS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR259(2)] to IRS1 (phospho Y632)
  • Specificityab109543 only detects IRS1 phosphorylated at Tyrosine 632.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Tyrosine 632 of Human IRS1.

  • Positive control
    • 293T cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
    • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
    • Sequence similaritiesContains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
      Phosphorylation of Tyr-896 is required for GRB2-binding.
    • Information by UniProt
    • Database links
    • Alternative names
      • HIRS 1 antibody
      • HIRS1 antibody
      • Insulin receptor substrate 1 antibody
      • IRS 1 antibody
      • IRS-1 antibody
      • IRS1 antibody
      • IRS1_HUMAN antibody
      • OTTHUMP00000164234 antibody
      see all

    Anti-IRS1 (phospho Y632) antibody [EPR259(2)] images

    • All lanes : Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543) at 1/1000 dilution

      Lane 1 : 293T cell lysate, untreated
      Lane 2 : 293T cell lysate treated with Alkaline Phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 132 kDa

    References for Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)

    ab109543 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"