Overview

  • Product nameAnti-IRS1 (phospho Y896) antibody
    See all IRS1 primary antibodies
  • Description
    Rabbit polyclonal to IRS1 (phospho Y896)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from a region of IRS 1 that contains tyrosine 896. The sequence is conserved in human, mouse, and rat.

  • Positive control
    • Chinese Hamster Ovary cell line transfected with a vector encoding the human insulin receptor (CHO-T) and transiently transfected with a vector encoding IRS 1 and stimulated with insulin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab4873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 165 kDa.Can be blocked with IRS1 (phospho Y896) peptide (ab5224).

Target

  • FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • Sequence similaritiesContains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • Post-translational
    modifications
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

Anti-IRS1 (phospho Y896) antibody images



  • Predicted band size : 165 kDa


    Peptide Competition: Extracts prepared from CHO-T cells transiently transfected with human IRS-1 and treated with 50 nM insulin for 5 minutes were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4873 antibody, following prior incubation with: no peptide (1), a generic phosphotyrosine containing peptide (2), the non-phosphopeptide corresponding to the immunogen (3), or the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4873 blocks the antibody signal, thereby demonstrating the specificity of the antibody.

    Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with human IRS 1 and treated with 50 nM insulin for 5 minutes were resolved by SDSPAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either not treated (lanes 5, 7) or treated (lanes 6, 8) with CD45, a tyrosine phosphatase, then incubated with pan antibody (lanes 5, 6) or with 0.50 µg/mL ab4873 antibody (lanes 7, 8). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that the amount of total IRS 1 is equal before and after phosphatase stripping, and that ab4873 antibody produces a specific signal.

References for Anti-IRS1 (phospho Y896) antibody (ab4873)

This product has been referenced in:
  • Stuart CA  et al. Insulin resistance and muscle insulin receptor substrate-1 serine hyperphosphorylation. Physiol Rep 2:N/A (2014). Read more (PubMed: 25472611) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"