The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Detects a band of approximately 170 kDa (predicted molecular weight: 132 kDa).
Application notesIs unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
FunctionMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
Involvement in diseasePolymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
Post-translational modificationsSerine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding.
References for Anti-IRS1 (phospho Y896) antibody [EP260Y] (ab46800)
This product has been referenced in:
Weng SW et al. Study of insulin resistance in cybrid cells harboring diabetes-susceptible and diabetes-protective mitochondrial haplogroups. MitochondrionN/A:N/A (2013).
Read more (PubMed: 23948373) »
Liu S et al. Antisense treatment of IGF-IR enhances chemosensitivity in squamous cell carcinomas of the head and neck. Eur J Cancer46:1744-51 (2010).
Read more (PubMed: 20451373) »