Overview

  • Product nameAnti-IRS2 antibody [EP903Y]
    See all IRS2 primary antibodies
  • Description
    Rabbit monoclonal [EP903Y] to IRS2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human IRS2.

  • Positive control
    • 293 cell lysate + insulin; human cervical carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab46811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Predicted molecular weight: 137 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay mediate the control of various cellular processes by insulin.
    • Sequence similaritiesContains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP903Y] images

    • Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin

      Secondary
      Goat-anti rabbit, HRP labelled, at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).
      The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
    • This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.

    References for Anti-IRS2 antibody [EP903Y] (ab46811)

    This product has been referenced in:
    • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab46811.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"