• Product nameAnti-IRS2 antibody [EP976Y]
    See all IRS2 primary antibodies
  • Description
    Rabbit monoclonal [EP976Y] to IRS2
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Ser1203 of human IRS2.

  • Positive control
    • 293 cell lysate; human kidney tissue. IF/ICC: SKNSH cell line.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab52606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionMay mediate the control of various cellular processes by insulin.
    • Sequence similaritiesContains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP976Y] images

    • ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µg

      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.

    References for Anti-IRS2 antibody [EP976Y] (ab52606)

    ab52606 has not yet been referenced specifically in any publications.

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