Overview

  • Product name
    Anti-IRS2 antibody [EP976Y]
    See all IRS2 primary antibodies
  • Description
    Rabbit monoclonal [EP976Y] to IRS2
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Ser1203 of human IRS2.

  • Positive control
    • 293 cell lysate; human kidney tissue. IF/ICC: SKNSH cell line.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab52606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      May mediate the control of various cellular processes by insulin.
    • Sequence similarities
      Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Cytoplasm > cytosol.
    • Information by UniProt
    • Database links
    • Alternative names
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Images

    • ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.

    References

    This product has been referenced in:
    • Sasaki N  et al. Ganglioside GM1 Contributes to the State of Insulin Resistance in Senescent Human Arterial Endothelial Cells. J Biol Chem 290:25475-86 (2015). WB . Read more (PubMed: 26338710) »
    • El-Ami T  et al. A novel inhibitor of the insulin/IGF signaling pathway protects from age-onset, neurodegeneration-linked proteotoxicity. Aging Cell 13:165-74 (2014). Read more (PubMed: 24261972) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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