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Synthetic peptide (YKASSpPAESS), corresponding to amino acids 727-736 of human IRS2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 719-728 of mouse IRS2 at the phosphorylation site of Serine 723 and to amino acids 229-238 of rat IRS2 at the serine phosphorylation site 233.
Our Abpromise guarantee covers the use of ab3690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 200 kDa. in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gelelectrophoresis.|
|IP||Use at an assay dependent concentration. Use at a concentration of 3.0 - 5.0 µg / extract from 107 cells.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-P||Use a concentration of 2 µg/ml.|