The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/1562500.
RelevanceIRX1 is a member of the Iroquois homeobox gene family. The genes from this family have multiple roles in vertebrate development. The gene encoding IRX1 is expressed in the brain, lung and gut during specific stages of mouse development.