Overview

  • Product name
  • Description
    Mouse polyclonal to IRX2
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length (Human) IRX2, (NP_150366.1).

  • Positive control
    • Hela cells and IRX2 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 10 µg/ml.
    Protein A purify ab72975 before starting ICC/IF procedure.
    WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities
      Belongs to the TALE/IRO homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Homeodomain protein IRXA2 antibody
      • Iroquois homeobox 2 antibody
      • Iroquois homeobox protein 2 antibody
      • Iroquois-class homeodomain protein irx-2 antibody
      • IRX 2 antibody
      • irx2 antibody
      • IRX2_HUMAN antibody
      • IrxA2 antibody
      see all

    Images

    • All lanes : Anti-IRX2 antibody (ab72975) at 1/500 dilution

      Lane 1 : IRX2 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
    • HeLa cells with Protein A purified ab72975 at 10ug/ml.

    References

    ab72975 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab72975.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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