Anti-IRX4 antibody (ab123542)
Key features and details
- Rabbit polyclonal to IRX4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IRX4 antibody -
Description
Rabbit polyclonal to IRX4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Macaque monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human IRX4 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139538) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
-
Function
Likely to be an important mediator of ventricular differentiation during cardiac development. -
Tissue specificity
Predominantly expressed in cardiac ventricles. -
Sequence similarities
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 373974 Chicken
- Entrez Gene: 50805 Human
- Entrez Gene: 50916 Mouse
- Entrez Gene: 306655 Rat
- Omim: 606199 Human
- SwissProt: Q9YGS0 Chicken
- SwissProt: P78413 Human
- SwissProt: Q2NL64 Human
see all -
Alternative names
- Homeodomain protein IRXA3 antibody
- Iroquois homeobox protein 4 antibody
- Iroquois-class homeodomain protein IRX-4 antibody
see all
Images
-
Anti-IRX4 antibody (ab123542) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab123542 has been referenced in 2 publications.
- Jia Z et al. 1,25-dihydroxyvitamin D3 signaling-induced decreases in IRX4 inhibits NANOG-mediated cancer stem-like properties and gefitinib resistance in NSCLC cells. Cell Death Dis 11:670 (2020). PubMed: 32820157
- Ward MC & Gilad Y A generally conserved response to hypoxia in iPSC-derived cardiomyocytes from humans and chimpanzees. Elife 8:N/A (2019). PubMed: 30958265