Overview

  • Product name
  • Description
    Rabbit polyclonal to IRX5
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Zebrafish, Macaque monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human IRX5.

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: HEK293; HeLa; PC3; DU145.

Properties

Applications

Our Abpromise guarantee covers the use of ab100939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Establishes the cardiac repolarization gradient by its repressive actions on the KCND2 potassium-channel gene. Required for retinal cone bipolar cell differentiation. May regulate contrast adaptation in the retina and control specific aspects of visual function in circuits of the mammalian retina (By similarity). Could be involved in the regulation of both the cell cycle and apoptosis in prostate cancer cells.
  • Sequence similarities
    Belongs to the TALE/IRO homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homeodomain protein IRX-2A antibody
    • Homeodomain protein IRXB2 antibody
    • Iroquois homeobox protein 5 antibody
    • Iroquois-class homeodomain protein IRX-5 antibody
    • IRX2A antibody
    • Irx5 antibody
    • IRX5_HUMAN antibody
    • IRXB2 antibody
    see all

Images

  • All lanes : Anti-IRX5 antibody (ab100939) at 1/250 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 4 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab100939 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab100939.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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