• Product nameAnti-ISG15 antibody [3E5]
    See all ISG15 primary antibodies
  • Description
    Mouse monoclonal [3E5] to ISG15
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human ISG15 (1-157aa) purified from E. coli.



Our Abpromise guarantee covers the use of ab48020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/1000 - 1/2000. Predicted molecular weight: 18 kDa.


  • FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • Sequence similaritiesContains 2 ubiquitin-like domains.
  • Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all

References for Anti-ISG15 antibody [3E5] (ab48020)

This product has been referenced in:
  • Dragin L  et al. Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation and high deoxynucleoside triphosphates supply. Retrovirology 10:30 (2013). WB ; Human . Read more (PubMed: 23497353) »
  • Murray EJ  et al. Knockdown of USP18 increases alpha 2a interferon signaling and induction of interferon-stimulating genes but does not increase antiviral activity in Huh7 cells. Antimicrob Agents Chemother 55:4311-9 (2011). WB ; Human . Read more (PubMed: 21709085) »

See all 2 Publications for this product

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