• Product nameAnti-ISG15 antibody
    See all ISG15 primary antibodies
  • Description
    Rabbit polyclonal to ISG15
  • Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant ISG15(Human).

  • Positive control
    • Most human cell lysates can be used as a positive control without induction or stimulation.
  • General notesCentrifuge product if not completely clear after standing at room temperature.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process, which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab14374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Detects a band of approximately 18.5 kDa.
ELISA 1/2000 - 1/10000.
IHC-Fr Use at an assay dependent concentration. PubMed: 20186858


  • FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
    In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
  • Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
  • Sequence similaritiesContains 2 ubiquitin-like domains.
  • Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • G1P2 antibody
    • hUCRP antibody
    • IFI 15 antibody
    • IFI15 antibody
    • Interferon alpha inducible protein antibody
    • Interferon induced protein 15 antibody
    • Interferon induced protein IFI-15K antibody
    • Interferon stimulated protein 15 kDa antibody
    • Interferon, alpha-inducible protein (clone IFI-15K) antibody
    • Interferon-induced 15 kDa protein antibody
    • Interferon-induced 17 kDa protein antibody
    • Interferon-induced 17-kDa/15-kDa protein antibody
    • IP17 antibody
    • ISG 15 antibody
    • ISG15 antibody
    • ISG15 ubiquitin like modifier antibody
    • ISG15_HUMAN antibody
    • Ubiquitin cross-reactive protein antibody
    • Ubiquitin-like protein ISG15 antibody
    • UCRP antibody
    see all

Anti-ISG15 antibody images

  • Anti-ISG15 antibody (ab14374) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 12 minutes

References for Anti-ISG15 antibody (ab14374)

This product has been referenced in:
  • Hirashima K  et al. Telomere length influences cancer cell differentiation in vivo. Mol Cell Biol 33:2988-95 (2013). ICC/IF . Read more (PubMed: 23716593) »
  • Salajegheh M  et al. Interferon-stimulated gene 15 (ISG15) conjugates proteins in dermatomyositis muscle with perifascicular atrophy. Ann Neurol 67:53-63 (2010). IHC-Fr ; Human . Read more (PubMed: 20186858) »

See all 3 Publications for this product

Product Wall

Thank you for your enquiry. No, I do not know of anyone that did not see these extra bands. It is expected to see these bands. My understanding is that these extra bands come from the binding of other proteins to ISG15 and that these proteins are ...

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Thank you for your phone call. Yes, ab14374 and ab14687 are the same antibody. I have removed ab14687 from our list and so you can go ahead and order ab14374. Please let me know if you have any additional questions.