Overview

  • Product nameAnti-ISG15 antibody
    See all ISG15 primary antibodies
  • Description
    Rabbit polyclonal to ISG15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human ISG15 protein with an N-terminal tag (expressed in E. coli)

Properties

Applications

Our Abpromise guarantee covers the use of ab45285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Predicted molecular weight: 18 kDa. This antibody was tested against the recombinant protein used as immunogen. We have no data regarding the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
      In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
    • Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
    • Sequence similaritiesContains 2 ubiquitin-like domains.
    • Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • G1P2 antibody
      • hUCRP antibody
      • IFI 15 antibody
      • IFI15 antibody
      • Interferon alpha inducible protein antibody
      • Interferon induced protein 15 antibody
      • Interferon induced protein IFI-15K antibody
      • Interferon stimulated protein 15 kDa antibody
      • Interferon, alpha-inducible protein (clone IFI-15K) antibody
      • Interferon-induced 15 kDa protein antibody
      • Interferon-induced 17 kDa protein antibody
      • Interferon-induced 17-kDa/15-kDa protein antibody
      • IP17 antibody
      • ISG 15 antibody
      • ISG15 antibody
      • ISG15 ubiquitin like modifier antibody
      • ISG15_HUMAN antibody
      • Ubiquitin cross-reactive protein antibody
      • Ubiquitin-like protein ISG15 antibody
      • UCRP antibody
      see all

    Anti-ISG15 antibody images

    • All lanes : Anti-ISG15 antibody (ab45285) at 1/1000 dilution

      Lane 1 : Negative control (same fusion tag, different protein)
      Lane 2 : Recombinant Human ISG15 (immunogen)


      Predicted band size : 18 kDa

    References for Anti-ISG15 antibody (ab45285)

    This product has been referenced in:
    • Franzi S  et al. Type 1 interferons inhibit myotube formation independently of upregulation of interferon-stimulated gene 15. PLoS One 8:e65362 (2013). WB . Read more (PubMed: 23750257) »
    • Liao AP  et al. Human plasmacytoid dendritic cell accumulation amplifies their type 1 interferon production. Clin Immunol 136:130-8 (2010). WB . Read more (PubMed: 20346735) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us. So far, the antibody has only been tested on human samples in WB. Mouse has not yet been tested. How well it might work with mouse would depend on the sequence homology between mouse and human. I have aligned the hum...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"