Overview

  • Product nameAnti-ISG15 antibody [EPR3445]
    See all ISG15 primary antibodies
  • Description
    Rabbit monoclonal [EPR3445] to ISG15
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human ISG15.

  • Positive control
    • HeLa treated with IFN a and Daudi cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab92345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250.
WB 1/2000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections.
      In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
    • Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
    • Sequence similaritiesContains 2 ubiquitin-like domains.
    • Cellular localizationCytoplasm. Secreted. UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • G1P2 antibody
      • hUCRP antibody
      • IFI 15 antibody
      • IFI15 antibody
      • Interferon alpha inducible protein antibody
      • Interferon induced protein 15 antibody
      • Interferon induced protein IFI-15K antibody
      • Interferon stimulated protein 15 kDa antibody
      • Interferon, alpha-inducible protein (clone IFI-15K) antibody
      • Interferon-induced 15 kDa protein antibody
      • Interferon-induced 17 kDa protein antibody
      • Interferon-induced 17-kDa/15-kDa protein antibody
      • IP17 antibody
      • ISG 15 antibody
      • ISG15 antibody
      • ISG15 ubiquitin like modifier antibody
      • ISG15_HUMAN antibody
      • Ubiquitin cross-reactive protein antibody
      • Ubiquitin-like protein ISG15 antibody
      • UCRP antibody
      see all

    Anti-ISG15 antibody [EPR3445] images

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining ISG15 with ab92345 at 1/100 dilution.

    • All lanes : Anti-ISG15 antibody [EPR3445] (ab92345) at 1/2000 dilution

      Lane 1 : Hela cell lysate treated with IFN α
      Lane 2 : Daudi cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 15 kDa
      Observed band size : 15 kDa

    References for Anti-ISG15 antibody [EPR3445] (ab92345)

    This product has been referenced in:
    • Kim K  et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One 8:e81734 (2013). WB ; Human . Read more (PubMed: 24312579) »
    • Pal S  et al. Influenza A virus interacts extensively with the cellular SUMOylation system during infection. Virus Res 158:12-27 (2011). WB ; Human . Read more (PubMed: 21376763) »

    See all 2 Publications for this product

    Product Wall

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"