The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/250.
1/2000 - 1/5000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
1/100 - 1/250.
Application notesIs unsuitable for Flow Cyt or IP.
FunctionUbiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy.
Tissue specificityDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining ISG15 with ab92345 at 1/100 dilution.
Western blot - ISG15 antibody [EPR3445] (ab92345)
All lanes : Anti-ISG15 antibody [EPR3445] (ab92345) at 1/2000 dilution
Lane 1 : Hela cell lysate treated with IFN α Lane 2 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Secondary goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 15 kDa Observed band size : 15 kDa
References for Anti-ISG15 antibody [EPR3445] (ab92345)
This product has been referenced in:
Kim K et al. Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex. PLoS One8:e81734 (2013).
Read more (PubMed: 24312579) »
Pal S et al. Influenza A virus interacts extensively with the cellular SUMOylation system during infection. Virus Res158:12-27 (2011).
Read more (PubMed: 21376763) »