Overview

  • Product name
  • Description
    Rabbit polyclonal to ISG20
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-181 of Human ISG20 (Q96AZ6).

  • Positive control
    • Raji whole cell lysate. HeLa cells. BT483 xenograft.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 20 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses.
  • Tissue specificity
    Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung.
  • Sequence similarities
    Belongs to the exonuclease superfamily.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD25 antibody
    • Estrogen regulated transcript 45 protein antibody
    • Estrogen-regulated transcript 45 protein antibody
    • HEM45 antibody
    • Interferon stimulated exonuclease gene 20kDa antibody
    • Interferon stimulated gene 20 kDa protein antibody
    • Interferon-stimulated gene 20 kDa protein antibody
    • Isg20 antibody
    • ISG20_HUMAN antibody
    • Promyelocytic leukemia nuclear body associated protein antibody
    • Promyelocytic leukemia nuclear body-associated protein ISG20 antibody
    see all

Images

  • Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human non-Hodgkin lymphoma tissue sections, labelling ISG20 with ab154393 at a dilution of 1/50 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with citrate buffer at pH 6.0 incubated for 15 minutes. Blocking was a with 5% serum incubated for 30 minutes at 25°C. Secondary was a Goat anti-rabbit polyclonal CY3® conjugate at 1/200. Counterstain was with DAPI against nuclear DNA.

    See Abreview

  • Anti-ISG20 antibody (ab154393) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 20 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded BT483 xenograft, labelling ISG20 with ab154393 at 1/500 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling ISG20 with ab154393 at a 1/500 dilution. Co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Zheng Z  et al. Estradiol and proinflammatory cytokines stimulate ISG20 expression in synovial fibroblasts of patients with osteoarthritis. Intractable Rare Dis Res 6:269-273 (2017). Read more (PubMed: 29259855) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human tumor lymph node (non-Hodgkin lymphoma, T ce)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0, 15 min
Permeabilization
No
Specification
Human tumor lymph node (non-Hodgkin lymphoma, T ce
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Dr. Assia Angelova

Verified customer

Submitted Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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